The Electropolymerization of Conductive Polymer Ppy-PANi on Gold Electrodes for Uric Acid Biosensor

Conducting polymers have emerged as potential candidates for biosensors. Due to their straightforward preparation methods, unique properties, and stability in air, conducting polymers have been applied to energy storage, electrochemical devices, memory devices, biosensors, and electro catalysts. Many research studies have been conducted on the use of conjugated polymers in the construction of chemical sensors including potentiometric, conduct metric and amperometric sensors or biosensors over the last decade. This paper will describe the galvanostatic electropolymerization of conducting polymer polypyrrole-polyaniline on gold electrodes of uric acid biosensor. The combined layer of conducting polymer Ppy-PANi will be used as immobilization interfacial membrane of uricase enzyme to increase the sensitivity and response of uric acid biosensor. The gold electrodes has been fabricated using thick film technology on alumina substrates. The characterization of uricase has been evaluated as preliminary process before the enzyme immobilized on the surface of conducting polymer Ppy-PANi. Index Term-Conducting polymer, electropolymerization, biosensor, thick film technology.